The scientists discovered chemical markers of DNA in the calcified cartilage of duck-billed dinosaurs in the 1980s. In the 1980s, paleontologists found dinosaur nesting grounds with dozens of nests in northern Montana.
And identified them as Hypacrosaurus stabingeri, which lived 75 million years ago. Now, a team of researchers from the United States, Canada.
And China has investigated the molecular innervation of calcified cartilage in one of the Hypacrosaurus stabingii nestled at the extracellular, extracellular and intracellular levels.
They were found to be conserved fragments of proteins and chromosomes in dinosaur chondrocytes (cartilage cells), a chemical marker of DNA. The findings further support the idea that these basic molecules can persist for millions of years.
“The bones of dinosaur skulls do not fuse when inserted, but some of them contain cartilaginous plaques, which then fuse as bones between them,” said Dr. Said Elida Baileul.
Apaleontologist at the Institute of Vertebrate Paleontology . And Paleoanthropology and the Center of Excellence in Life and Paleoenvironments, Chinese Academy of Sciences.
“Seeing specifically conserved microstructures that resembled only the specific type of cell found in cartilage, and that were present in the living organism in these tissues, led us to raise the hypothesis that cell conservation is at the molecular level. It can grow to” .
Dr. Baileul and his colleagues performed an immunological and histochemical analysis of the skull tissues of the Hypacrosaurus stabingeri calf and compared the results of the emu skull at the same stage of development.
“Bird skulls are skulls, or stern, in the same pattern as dinosaur skulls, and primitive birds (mice) like emus are the closest relatives we have today to non-avian dinosaurs,” the Professor Mary Schweitzer..Said.
North Carolina State University, Natural Sciences Museum of North Carolina and Lund University. Cartilaginous tissues and chondrocytes of the dinosaur skull reacted with antibodies against collagen II.
The comparison of the results with EMU confirms the conclusion. Chondrocytes isolated from Hypacrosaurus stabingeri and their positive response to two DNA assays: (A, B, E)..
Chondrocytes isolated from Hypacrosaurus stabingeri and MS are photographed under transmitted light (green arrow).Hypacrosaurus stebingeri chondrocytes were successfully isolated as individual cells (A) and cell doublets (B); Hypacrosaurus stebingeri (C) and emu chondrocytes (F) that show a positive response to propidium iodide.
And a DNA interlocking dye, in a small, spherical region that detects intracellular (white arrow); Hypacrosaurus stebingeri (D) and emu chondrocytes (G) also show a similar binding.
When exposed to 4”-dimidino-2-phenylindole dihydrochloride, another specific DNA stain (black arrow), although in both cases, cell staining Emu. Much more than dinosaur cells. Image by Beliul et al, doi: 10.1093 / nsr / nwz206.
“These tests show how specific the antibodies are for each type of protein and support the presence of collagen II in these tissues,” said Professor Schweitzer. In addition, bacteria cannot produce collagen, which controls pollution as a source of molecules.
The scientists also tested the presence of chemical markers compatible with the DNA in the microstructure by two complementary histochemical stains that bind DNA fragments into the cells: 4 ‘, 6’-dimidino-2-phenylindole diodocloride and propidium iodide.
These chemical markers reacted with isolated cartilaginous cells, supporting the idea that some aromatic DNA can reside within the cells.
“We use two different types of interleaved stains, one of which will only attach a piece of DNA to dead cells, and the other that binds any DNA,” said Professor Schweitzer.
The staining points show reactivity, which means that they are bound to specific molecules within the microstructure and do not spread throughout the ‘cell’ as would be expected if they originated from bacterial contamination.
Although bone cells have previously been isolated from dinosaur bone, this is the first time that cartilage producing cells have been isolated from fossils, said Dr. Baileul.
“This is an exciting discovery that adds to the growing body of evidence that these tissues, cells and nuclear material can persist for millions. The findings were published in the National Science Review Journal.